Potato Dextrose Agar is recommended by the American Public Health Association for plate counts of yeasts and molds in the examination of foods and dairy products. It is also used for maintenance, cultivation and sporulation of stock cultures of various dermatophytes and for differentiation of atypical varieties of dermatophytes by pigment production.
Potato starch infusion and dextrose support luxuriant growth and spore stimulation of fungi. It is recommended for use in combination with InTray DM-FungID and InTray SAB-FungID w/CC for microscopy and morphological observations.
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Incubate inoculated trays in a dark humidified environment for up to 21 days at 25-30°C. Observe the tray(s) daily through the clear viewing window
For isolation of fungi causing systemic mycoses, two sets of media should be inoculated:
Expiration is 12 months past the date of manufacture.
Upon receipt, store InTray PDA-FungID at 2-25°C. Avoid freezing or prolonged storage at temperatures above 40°C. Do not open until ready to use. Do not use if the medium shows signs of deterioration or contamination.
If a fungal infection is strongly suspected and the culture test result is negative, it may be appropriate to retest giving more care to specimen collection. Optimally, simultaneously inoculate samples on both InTray DM-FungID & InTray SAB-FungID without antimicrobials (e.g., for some pathogenic fungi inhibited by antimicrobials)
Transfer of fungal growth to slide examination: Stained slide preparations can be made as appropriate based on 10x Obj. examination of InTray SAB-FungID. InTray Potato Dextrose Agar (PDA) can be used to induce sporulation