InTray SAB-FungID w/ CC contains Sabouraud’s dextrose agar with chloramphenicol and cycloheximide, a selective medium used to aid in the detection of dermatophyte fungi from clinical specimens with mixed microbiota.
InTray SAB-FungID w/ CC is a dynamic system with built-in components and features that are designed for user compatibility and ease of dermatophyte fungi detection (i.e., 10x objective microscopy direct from the tray). The medium allows for the growth and observation of distinct colony morphology and color (i.e., pigments) of dermatophyte fungi, while inhibiting most grampositive bacteria, gram-negative bacteria, yeast and saprophytic fungi. Dermatophytes are fungi in the genera Microsporum, Trichophyton and Epidermophyton. They are capable of metabolizing keratin found in skin, hair and nails of living hosts.
The fungi characteristically may invade the cutaneous tissue of the living host but rarely penetrate the subcutaneous tissue. Tinea and ringworm are two terms commonly used to describe dermatophytes.
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Incubate inoculated trays in a dark environment for up to 21 days at 25-30°C. Observe the trays daily through the clear viewing window
InTray SAB-FungID w/ CC has an expiration of 12 months from date of manufacture.
On receipt, store trays at 2-25°C in the dark. Avoid freezing or prolonged storage at temperatures greater than 40°C. Do not use trays if the medium shows signs of deterioration or contamination.
If a fungal infection is strongly suspected and the culture test result is negative, it may be appropriate to retest giving more care to specimen collection. Optimally, simultaneously inoculate samples on both InTray DM-FungID & InTray SAB-FungID without antimicrobials (e.g., for some pathogenic fungi inhibited by antimicrobials)
Transfer of fungal growth to slide examination: Stained slide preparations can be made as appropriate based on 10x Obj. examination of InTray SAB-FungID. InTray PDA-FungID can be used to induce sporulation.
Singh S, Beena P M. Comparative study of different microscopic techniques and culture media for the isolation of dermatophytes. Indian J Med Microbiol [serial online] 2003 [cited 2019 May 9 ];21:21-24
Available from: http://www.ijmm.org/text.asp?2003/21/1/21/8310
Diagnostic microbiology in veterinary dermatology: Present and Future