InTray® COLOREX™ Screen

Experience Chromogenic Differentiation

InTray® COLOREX™ ScreenInTray® COLOREX™ Screen
Quick Nav

Categories

InTray® COLOREX™ Screen

Card image cap
For Rapid Isolation and Differentiation of Urinary Tract Pathogens

The major target of this medium is the detection of urinary tract pathogens, but COLOREX Screen has a broader application as a general nutrient agar for the isolation of various microorganisms.

COLOREX Screen can also be used to differentiate various microorganisms in other infected areas; e.g., scars, wounds, etc. In addition, COLOREX Screen is useful when supplemented with various antibiotics in detecting increasingly important nosocomial and multidrug resistant microorganisms.

For chromogenic InTrays with antibiotic supplementals, please see:

COLOREX ESBL      COLOREX KPC

 

Specifications

Accurate Detection

E. coli Enterococcus Klebsiella Enterobacter Citrobacter Proteus Pseudomonas Staph aureus Staph saprophyticus

Time to Result

Incubate at 37°C for 24-72 hours under ambient atmosphere. Note any colony color and morphology

Storage

Expiration is 12 months from the date of manufacture. 

Upon receipt, store InTray COLOREX Screen under refrigeration (2-8°C). Medium can be kept for one day at  ambient temperature. Avoid freezing or prolonged storage at temperatures above 40°C. Do not open until ready to use. Do not use if the medium shows signs of deterioration or contamination.

Specimen

Urine   |   Pus   |   Stool   |   Scar tissue

Step-by-Step

Previous Next

More Information

The VTM-C19 Transit Tube contains a viral transport medium (VTM) intended to be inoculated with nasopharyngeal (NP) or oropharyngeal (OP) synthetic fiber swab specimens for transport to the lab.

VTM-C19 transport media is designed for specimen preservation in preparation for analysis with validated qRT-PCR assays to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes COVID-19 disease in humans.

This is CDC-approved media for COVID-19 sample collection and transport. VTM-C19 has been validated for stable transport of SARS-CoV-2 only.

Biomed Diagnostics also offers Saline .85% transport media for a general viral transport – our R&D lab has validated the Saline .85% for SARS-CoV-2, and the Saline is checked throughout the shelf life of the product for sterility by our QC Labs
The VTM-C19 was validated by our internal R&D team using qRT-PCR (N1 gene). Validation results confirmed that VTM C19 maintained the viability of the RNA for 72 hours post sample collection (when stored at 2-8°C. Cell lysate of SARS-Cov-2 infected cells were used for validation (not live virus).
Currently we are shipping VTM-C19 as a cold product
Biomed can provide the Validation report on an as-requested basis.
Internal validation (qRT-PCR) were conducted using Roche Light cycler, and the RNA was extracted using QIAGEN RNA extraction kit
Biomed’s VTM-C-19 has been validated to maintain the viral specimen at Room Temperature for up to 72 hours, after inoculation.
Current shelf life of the product is 5 months from the date of manufacture, in refrigerated storage conditions of 2-8°C. The product may be stored at Room Temperature for up to one month.

Room Temperature validation is ongoing and will be update monthly.
Biomed does not manufacture swabs; but we have a unique partnership and pricing with Rhino Diagnostics, to supply NP or OP swabs. Contact us for more information.
Practical Bench Comparison of BBL CHROMagar Orientation and Standard Two-Plate Media for Urine Culture
John Merlino, Steven Siarakas, Graham Robertson, Glenn Funnell, Thomas Gottlieb, and Ross Bradbury

J Clin Microbiol; 34.

Read Paper ►

 

Evaluation of Use of a New Chromogenic Agar in Detection of Urinary Tract Pathogens
Z. Samra,M. Heifetz, J. Talmore, E. Bain, and J. Bahar

JOCM Vol 36, No 4

Read Paper ►

 

Evaluation of CHROMagar Orientation for Differentiation and Presumptive Identification of Gram-Negative Bacilli and Enterococcus Species
Z. Samra,M. Heifetz, J. Talmore, E. Bain, and J. Bahar

J Clin Microbiol; 34.

Read Paper ►

 

A Study on Evaluation of Different Culture Media for the Isolation of Routine Urinary Pathogens
Biji, S. Manjusree, O. Sasikumari and J.T. Ramani Bai/h6>

IJCM Vol 6 No 2

Read Paper ►
This test was performed to evaluate the Biomed Saline Solution (0.85%) by detection of heat inactivated cell lysate from SARS-Cov-2 (ATCC® VR-1986HK™) infected cells, after RNA was isolated using the QIAamp® Viral RNA Mini Kit (QIAGEN®). Detection of the isolated SARSCov-2 RNA was by qRT-PCR using New England Biolabs® OneTaq® One-Step RT-PCR kit and run on a Roche® Lightcycler® 96 with EvaGreen® (Biotium®) detection from samples after storage of viral lysates in Saline Solution (0.85%) at 24, 48 and 72 hours at room temperature (25°C). Synthetic RNA standard (BEI Resources) at 2.9 x 108 was serially (10-fold) diluted for the standard (STD) in the amplification reaction.

 

The assay results demonstrate consistent amplification and Cq quantification of the nCov-2 N gene (using the N1 CDC primer) in the solution after incubations. In addition, excellent dilution linearity is demonstrated across all samples and replicates (See Figure 1) indicating consistency in the performance of the solution Saline Solution (0.85%) replicate samples and incubation time. This data indicates that the Biomed ISO 13845 manufactured Saline Solution (0.85%) does not negatively interfere with the qRT-PCR detection of Sars-Cov-2 viral nucleic acid materials after incubation at room temperature 25°C for 72 hours. A delay in amplification signal was observed after 168-hour (7 days) incubation at 25°C, probably due to specimen degradation. However, incubation at 4°C for 168 hours (7 days) did not negatively interfere with the qRT-PCR performance. In conclusion, the result indicates that the Saline Solution (0.85%) is compatible with the designated viral specimen inoculation, nucleic acid extraction and qRT–PCR assay when used as described herein.
 

All samples shown are means from triplicate assays, with error bars indicating the arithmetic error for Cq shown. The performance of the qRT-PCR reaction assessed with standard curves indicated R2 of 0.98, Efficiency of 114%, and Slope of -3.033. All the values fall within acceptable quantitative PCR ranges.

Card image cap