The major target of this medium is the detection of urinary tract pathogens, but COLOREX Screen has a broader application as a general nutrient agar for the isolation of various microorganisms.
COLOREX Screen can also be used to differentiate various microorganisms in other infected areas; e.g., scars, wounds, etc. In addition, COLOREX Screen is useful when supplemented with various antibiotics in detecting increasingly important nosocomial and multidrug resistant microorganisms.
J Clin Microbiol; 34.
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JOCM Vol 36, No 4
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J Clin Microbiol; 34.
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IJCM Vol 6 No 2
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The assay results demonstrate consistent amplification and Cq quantification of the nCov-2 N gene (using the N1 CDC primer) in the solution after incubations. In addition, excellent dilution linearity is demonstrated across all samples and replicates (See Figure 1) indicating consistency in the performance of the solution Saline Solution (0.85%) replicate samples and incubation time. This data indicates that the Biomed ISO 13845 manufactured Saline Solution (0.85%) does not negatively interfere with the qRT-PCR detection of Sars-Cov-2 viral nucleic acid materials after incubation at room temperature 25°C for 72 hours. A delay in amplification signal was observed after 168-hour (7 days) incubation at 25°C, probably due to specimen degradation. However, incubation at 4°C for 168 hours (7 days) did not negatively interfere with the qRT-PCR performance. In conclusion, the result indicates that the Saline Solution (0.85%) is compatible with the designated viral specimen inoculation, nucleic acid extraction and qRT–PCR assay when used as described herein.
All samples shown are means from triplicate assays, with error bars indicating the arithmetic error for Cq shown. The performance of the qRT-PCR reaction assessed with standard curves indicated R2 of 0.98, Efficiency of 114%, and Slope of -3.033. All the values fall within acceptable quantitative PCR ranges.