Sabouraud Dextrose Agar (SAB) is a general-purpose medium devised by Sabouraud for the cultivation of pathogenic and non-pathogenic fungi, including dermatophytes. For SAB+PVG, the addition of the antimicrobics: Polymyxin B, Vancomycin and Gentamicin is a modification designed to increase fungal specificity by bacterial inhibition.
The low pH (approximately 5.6) is favorable for growth of fungi, especially yeasts and dermatophytes. SAB is slightly inhibitory to contaminating bacteria in clinical specimens.2-4 This inhibition is further enhanced in SAB+PVG. SAB is recommended in the Unites States Pharmacopoeia (USP) for use in performing total combined mold and yeast counts (Microbial Limits Tests).
For isolation of fungi from potentially contaminated specimens, best practice suggests InTray SAB+PVG should be run in parallel with the non-selective InTray SAB-FungID.
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Incubate For isolation of fungi from potentially contaminated specimens, a selective medium (e.g., SAB+PVG) should be inoculated in parallel with a non-selective medium (e.g., SAB). Incubate the InTray at 25-30°C for 18-48 hours, or up to 7-14 days for Trichophyton. Examine all cultures at least weekly for fungal growth. Samples should be held for 4-6 weeks before being reported as negative.
InTray SAB+PVG has an expiration of 12 months from the date of manufacture.
Upon receipt, store InTray SAB+PVG at 2-8°C. Avoid freezing or prolonged storage at temperatures above 40°C. Do not use InTray SAB+PVG if the medium shows signs of deterioration or contamination.