Sample Transport
Culture ☆ Result

Easy Identification

InTray® SMA

Quick Links: Specifications Detection Incubation Storage

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InTray SMA (Sorbitol MacConkey Agar) is a selective and differential media for the detection of sorbitol-non-fermenting Escherichia coli serotype O157:H7 associated with hemorrhagic colitis. This medium is also referred to as “MacConkey Sorbitol Agar”.

Escherichia coli serotype O157:H7 is a human pathogen associated with hemorrhagic colitis that results from the action of a shiga-like toxin. On standard MacConkey agar containing lactose, this strain is indistinguishable from other lactose-fermenting Escherichia coli. Field results show that the growth of Escherichia coli O157:H7 on SMA is heavy and occurs in almost pure culture — as colorless sorbitol-non-fermenting colonies. Most organisms of the fecal flora ferment sorbitol and appear pink on this medium. SMA, therefore, permits ready recognition of Escherichia coli O157:H7 in stool cultures.

Specifications

Product Principle

Durable injection molded device
Highly selective media designed for growth, transport and result
Sealed environment with Tyvek^® air filtration
Resealable media well reduces exposure and contamination

Simple Convenience

The InTray is designed for specimen collection/ inoculation at point-of-care
Saves time, material cost and waste, reducing exposure to collected sample
Place directly on the microscope tray for observation through the cassette’s clear anti-fog window
Selectively produces distinctive colony growth with typical identifiable morphology

Accurate Detection

Test Strain	ATCC #	Colony Appearance
E. coli O157:H7	35150	Colorless
E. coli	25922	Pink to Red w/ bile ppt.
E. faecalis	29212	Marked to complete inhibition

Time to Result

Incubate at 35 ± 2°C for 18-24 hours under ambient atmosphere.

Detection

ESBL Strains: K. pneumoniae & E. coli

Storage

InTray SMA has a shelf life of 12 months from the date of manufacture.

Upon receipt, store InTray SMA (Sorbitol MacConkey Agar) under refrigeration (2-8°C). Medium can be kept for one day at ambient temperature. Avoid freezing or prolonged storage at temperatures above 40°C. Do not open until ready to use. Do not use if the medium shows signs of deterioration or contamination.

Features and Benefits

Shelf-life and durability

Designed for long shelf life, durability and stackable for incubation, or 12 month storage.
No re-plate or wet mount
Once InTray is inoculated, no other culture preparation is required... saving time and cost.
Designed for specimen collection/inoculation at point-of-care, a fully enclosed system for transport, incubation, result and microscopic observation.
The sealed prepared media device is selectively formulated for accurate detection and identification of pathogens and non-pathogens