InTray COLOREX GBS agar is a chromogenic medium for differentiation and isolation of pure cultures of Streptococcus agalactiae (GBS). The test can be performed with samples of composed of mixed populations of bacteria, e.g., vaginal or rectal swabs, urine, feces, etc. COLOREX GBS is not intended for use in the identification of colonization with GBS to aid in the prevention and control of GBS in healthcare settings. COLOREX GBS is not intended to diagnose GBS infections, guide or monitor treatment for infections, or provide susceptibility results. Sub-culture is necessary for bacterial identification and susceptibility testing.
With COLOREX GBS medium, S. agalactiae, including non-haemolytic strains are distinguishable by colony color. High sensitivity and specificity of the medium lead to a higher detection rate of S. agalactiae spp. Selective agents inhibit the growth of Gram-positive organisms. Artificial substrates are metabolized by specific microbial enzymes liberating insoluble chromogenic compounds. S. agalactiae species produce deep pink to mauve colored colonies. Bacteria other than S.agalactiae spp. produce blue colonies or are colorless or inhibited. Enrichment of original samples in Todd Hewitt or LIM broth is possible and may give best results.
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Incubate at 35-37°C for 18-24 hours under ambient atmosphere.
Colonies of S. agalactiae spp. appear deep pink to mauve. Non S. agalactiae bacteria are blue, colorless or are inhibited. Latex agglutination testing can be performed directly from the colony.
InTray COLOREX GBS has a 6-month expiration date from the date of manufacturing.
Upon receipt, store InTray COLOREX GBS under refrigeration (2-8°C). Medium can be kept for one day at ambient temperature. Protect media from exposure to light, excessive heat, moisture and freezing. Do not open until ready to use. Do not use if the medium shows signs of deterioration, shrinking, cracking, discoloration or contamination