Sorbitol MacConkey Agar (SMA) is a selective and differential media for the detection of sorbitol-non-fermenting Escherichia coli serotype 0157:H7 associated with hemorrhagic colitis. This medium is also referred to as "MacConkey Sorbitol Agar."
E. coli serotype 0157:H7 is a human pathogen associated with hemorrhagic colitis that results from the action of a shiga-like toxin. On standard MacConkey Agar containing lactose, this strain is indistinguishable from other lactose-fermenting E. coli. Field results show that the growth of E. coli 0157:H7 on SMA is heavy and occurs in almost pure culture-- as colorless sorbitol-non-fermenting colonies. Most organisms of the fecal flora ferment sorbitol and appear pink on this medium. SMA, therefore, permits ready recognition of E. coli 0157:H7 in stool cultures.
100% specificity when used to isolate sorbitol-non-fermenting Escherichia coli serotype 0157:H7
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Incubate at 35 ± 2°C for 18-24 hours under ambient atmosphere.
InTray SMA has a shelf life of 12 months from the date of manufacture.
Upon receipt, store InTray SMA (Sorbitol MacConkey Agar) under refrigeration (2-8°C). Medium can be kept for one day at ambient temperature. Avoid freezing or prolonged storage at temperatures above 40°C. Do not open until ready to use. Do not use if the medium shows signs of deterioration or contamination.