Sample Transport
Culture ☆ Result

Easy Identification

E. coli O157:H7

InTray® SMA

Sorbitol MacConkey Agar (SMA) is a selective and differential media for the detection of sorbitol-non-fermenting Escherichia coli serotype 0157:H7 associated with hemorrhagic colitis. This medium is also referred to as "MacConkey Sorbitol Agar."

E. coli serotype 0157:H7 is a human pathogen associated with hemorrhagic colitis that results from the action of a shiga-like toxin. On standard MacConkey Agar containing lactose, this strain is indistinguishable from other lactose-fermenting E. coli. Field results show that the growth of E. coli 0157:H7 on SMA is heavy and occurs in almost pure culture-- as colorless sorbitol-non-fermenting colonies. Most organisms of the fecal flora ferment sorbitol and appear pink on this medium. SMA, therefore, permits ready recognition of E. coli 0157:H7 in stool cultures.

Specifications

Product Principle

Combine collection, culture, result and microscopic observation in one IVD device.
Highly selective prepared media in a device designed for in-vitro specimen transport and incubation.
Rapid presumptive-positive diagnosis without re-plating. Intray can be placed directly on microscope stage
Fully enclosed cassette with Tyvek® air filtration prevents contamination and exposure, post-collection.

Simple Convenience

The InTray is designed for specimen collection/ inoculation at point-of-care
Saves time, material cost and waste, reducing exposure to collected sample
Place directly on the microscope tray for observation through the cassette’s clear anti-fog window
Selectively produces distinctive colony growth with typical identifiable morphology

Accurate Detection

100% specificity when used to isolate sorbitol-non-fermenting Escherichia coli serotype 0157:H7

Test Strain	ATCC#	Color
E. coli O157:H7	35150	Colorless
E. coli	25922	Pink to red w/ bile ppt.
E. faecalis	29212	Marked to complete inhibition

Time to Result

Incubate at 35 ± 2°C for 18-24 hours under ambient atmosphere.

Storage

InTray SMA has a shelf life of 12 months from the date of manufacture.

Upon receipt, store InTray SMA (Sorbitol MacConkey Agar) under refrigeration (2-8°C). Medium can be kept for one day at ambient temperature. Avoid freezing or prolonged storage at temperatures above 40°C. Do not open until ready to use. Do not use if the medium shows signs of deterioration or contamination.

Features and Benefits

Shelf-life and durability

Designed for long shelf life, durability and stackable for incubation, or 12 month storage.
No re-plate or wet mount
Once InTray is inoculated, no other culture preparation is required... saving time and cost.
Designed for specimen collection/inoculation at point-of-care, a fully enclosed system for transport, incubation, result and microscopic observation.
The sealed prepared media device is selectively formulated for accurate detection and identification of pathogens and non-pathogens